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Root Cause Protocol

Gut Dysbiosis / SIBO

1. Root Cause Overview

Gut dysbiosis refers to a pathological imbalance in the composition, diversity, or metabolic activity of the intestinal microbiome. Small intestinal bacterial overgrowth (SIBO) is a specific form where colonic-type bacteria colonise the small intestine in excess, causing fermentation, malabsorption, and systemic immune activation. Both conditions disrupt the gut-immune-brain axis and are implicated in IBS, IBD, autoimmunity, mood disorders, and metabolic dysfunction. Key drivers include antibiotic overuse, proton pump inhibitor use, poor dietary fibre intake, chronic stress, and impaired motility.

2. Common Signs & Symptoms

  • Bloating, particularly after meals or carbohydrate intake
  • Excessive flatulence, belching
  • Alternating diarrhoea and constipation (IBS pattern)
  • Abdominal cramping and discomfort
  • Nutrient deficiencies (B12, iron, fat-soluble vitamins)
  • Brain fog and cognitive impairment
  • Skin conditions: rosacea, eczema, acne
  • Food intolerances (multiple, worsening over time)
  • Fatigue and low energy
  • Recurrent yeast infections or oral thrush

3. Labs to Consider

STANDARD LABS

CBC/CMP, ferritin/B12/vitamin D, stool testing when indicated, lactulose or glucose breath test for SIBO, celiac serology when appropriate.

FUNCTIONAL LAB OPTIONS

Choose the lab option based on what you want to assess within this root cause.

GI-MAP

Diagnostic Solutions

LAB
GI-MAP
COMPANY
Diagnostic Solutions
COST
~$350–$450
SAMPLE
Stool
KEY MARKERS
qPCR pathogens, H. pylori virulence factors, calprotectin, sIgA, anti-gliadin antibodies
Lab selection should be individualized based on clinical presentation and practitioner judgment.

4. Clinical Priorities

Confirm SIBO with appropriate testing before initiating antimicrobial protocols, and assess for contributors such as impaired motility, hypochlorhydria, prior infections, adhesions, medication effects, constipation, or structural issues. Use a phased approach: reduce bacterial load, support gut lining integrity, reintroduce beneficial flora as tolerated, and maintain progress with diet and lifestyle. Avoid prolonged antimicrobial use without reassessment.

Where to Start: Top 2 Supplements

1st Choice
Saccharomyces boulardii — 5–10 billion CFU/day

The single most evidence-backed probiotic for dysbiosis and SIBO. Antibiotic-resistant (safe to use alongside antimicrobial protocols), reduces pathogen load, restores sIgA, and protects the gut barrier. Start here before adding bacteria-based probiotics.

2nd Choice
Berberine — 500 mg TID

Broad-spectrum antimicrobial with the strongest evidence base among herbal agents for SIBO and dysbiosis. Simultaneously addresses pathogen overgrowth, reduces gut inflammation, and improves microbiome balance. Comparable to metronidazole in some studies.

5. Diet Strategy

During active dysbiosis/SIBO: consider a low-FODMAP diet (4–6 weeks) to reduce fermentable substrate. Emphasise easily digestible proteins and cooked vegetables. Avoid raw garlic, onions, legumes, and high-sugar foods during treatment phase. After treatment: gradually reintroduce prebiotic fibres and fermented foods (kefir, sauerkraut, kimchi) to rebuild microbiome diversity. Chew food thoroughly; avoid eating under stress.

6. Lifestyle Strategy

Optimise the migrating motor complex (MMC) by spacing meals 4–5 hours apart and avoiding snacking. Manage stress actively — the gut-brain axis is bidirectional and stress directly alters microbiome composition. Regular moderate exercise improves gut motility and microbiome diversity. Avoid unnecessary antibiotics and PPIs when possible. Address sleep quality — circadian disruption impairs gut barrier function.

7. Supplement Strategy

Full supplement list below. See Section 4 (Clinical Priorities) for the recommended Top 2 starting supplements. Add additional supplements based on lab results and clinical response at 4–6 week reassessment.

SupplementDosageIndicationEvidenceKey ResultsExample
Saccharomyces boulardii [1]
Generally well tolerated; avoid in immunocompromised patients or those with central venous catheters
5–10 billion CFU/day during and after antibiotic/antimicrobial treatmentPrevention of antibiotic-associated dysbiosis; Clostridioides difficile protection; gut immune supportSystematic review of RCTsSignificant reduction in antibiotic-associated diarrhoea and C. difficile recurrence; improved gut barrier markersJarrow Formulas Saccharomyces Boulardii + MOS
Berberine [2]
GI upset, constipation; contraindicated in pregnancy; may interact with CYP3A4 substrates
500 mg TID (with meals)Antimicrobial activity against SIBO-associated pathogens; gut microbiome rebalancingRCT and in vitro studiesSignificant reduction in pathogenic bacteria; improved IBS symptom scores; anti-inflammatory effects on gut mucosaThorne Berberine-500
Oregano Oil (Origanum vulgare) [3]
GI irritation; avoid in pregnancy; may alter gut flora broadly — use short-term with probiotic support
200 mg standardised extract (≥55% carvacrol) BIDBroad-spectrum antimicrobial; SIBO and pathogen eradication supportIn vitro and clinical studiesEffective against multiple GI pathogens including E. coli, Klebsiella, Candida; reduced SIBO-related symptomsNutricology Oregano Oil
Lactobacillus acidophilus + Bifidobacterium blend [4]
Transient bloating during first 1–2 weeks; avoid in immunocompromised without physician oversight
10–50 billion CFU/day (multi-strain)Microbiome restoration post-antimicrobial treatment; barrier function supportMeta-analysis of RCTsSignificant improvement in IBS symptoms, stool consistency, and gut permeability markersKlaire Labs Ther-Biotic Complete
Bacillus clausii [5]
Well tolerated; rare allergic reactions
2 billion spores TIDSpore-forming probiotic; antibiotic-resistant; SIBO and dysbiosis adjunctRCTReduced duration of acute diarrhoea; improved gut microbiome recovery post-antibiotic treatmentEnterogermina (Sanofi)
Garlic Extract (Allicin-standardised) [6]
GI upset, halitosis; anticoagulant interaction — caution with warfarin
600–900 mg/day standardised to allicinH. pylori eradication support; broad antimicrobial activityClinical and in vitro studiesInhibited H. pylori growth; reduced pathogenic bacterial load in GI tractAllimax 180 mg Allisure Powder

8. Safety Notes

Do not initiate antimicrobial protocols without confirmatory testing (breath test, stool PCR). Herbal antimicrobials can disrupt beneficial flora — always pair with probiotic support and follow with a re-inoculation phase. Refer for persistent symptoms, unexplained weight loss, rectal bleeding, or positive red-flag findings. SIBO frequently recurs without addressing the underlying motility or structural cause.

9. Citations & References

  1. [1]
    Saccharomyces boulardii
    McFarland LV. Systematic review and meta-analysis of Saccharomyces boulardii in adult patients. World J Gastroenterol. 2010;16(18):2202–2222.
    View source
  2. [2]
    Berberine
    Imenshahidi M, Hosseinzadeh H. Berberis vulgaris and berberine: an update review. Phytother Res. 2016;30(11):1745–1764.
    View source
  3. [3]
    Oregano Oil (Origanum vulgare)
    Force M, Sparks WS, Ronzio RA. Inhibition of enteric parasites by emulsified oil of oregano in vivo. Phytother Res. 2000;14(3):213–214.
    View source
  4. [4]
    Lactobacillus acidophilus + Bifidobacterium blend
    Ford AC, Quigley EMM, Lacy BE, et al. Efficacy of prebiotics, probiotics, and synbiotics in irritable bowel syndrome and chronic idiopathic constipation: systematic review and meta-analysis. Am J Gastroenterol. 2014;109(10):1547–1561.
    View source
  5. [5]
    Bacillus clausii
    Urdaci MC, Bressollier P, Pinchuk I. Bacillus clausii probiotic strains: antimicrobial and immunomodulatory activities. J Clin Gastroenterol. 2004;38(6 Suppl):S86–S90.
    View source
  6. [6]
    Garlic Extract (Allicin-standardised)
    Soffar SA, Mokhtar GM. Evaluation of the antiparasitic effect of aqueous garlic (Allium sativum) extract in hymenolepiasis nana and giardiasis. J Egypt Soc Parasitol. 1991;21(2):497–502.
    View source

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